The affinity of Digibind for digoxin is in the range of 10 9 to 10 11 M -1, which is greater than the affinity of digoxin for (sodium, potassium) ATPase, the presumed receptor for its toxic effects. 1 Ordinarily, following administration of Digibind, improvement in signs and symptoms of digitalis intoxication begins within one-half hour or less. 2 Experimental studies in animals indicate that these antibody fragments have a large volume of distribution in the extracellular space, unlike whole antibody which distributes in a space only about twice the plasma volume. 1 In humans with normal renal function, the half-life appears to be 15 to 20 hours. Related/similar drugs digoxin immune fab Digibind - Clinical PharmacologyĪfter intravenous injection of Digoxin Immune Fab (Ovine) in the baboon, digoxin-specific Fab fragments are excreted in the urine with a biological half-life of about 9 to 13 hours. The vial contains no preservatives.ĭigibind is administered by intravenous injection after reconstitution with Sterile Water for Injection (4 mL per vial).

These antibody fragments have a molecular weight of approximately 46,200.Įach vial, which will bind approximately 0.5 mg of digoxin (or digitoxin), contains 38 mg of digoxin-specific Fab fragments derived from sheep plus 75 mg of sorbitol as a stabilizer and 28 mg of sodium chloride. The antibody is then papain-digested and digoxin-specific Fab fragments of the antibody are isolated and purified by affinity chromatography. Sheep are immunized with this material to produce antibodies specific for the antigenic determinants of the digoxin molecule.

Production of antibodies specific for digoxin involves conjugation of digoxin as a hapten to human albumin. Digibind, Digoxin Immune Fab (Ovine), is a sterile lyophilized powder of antigen binding fragments (Fab) derived from specific antidigoxin antibodies raised in sheep.

Praxbind mechanism of action